A convenient spectrophotometric assay for the determination of l-ergothioneine in blood
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منابع مشابه
A convenient spectrophotometric assay for the determination of L-ergothioneine in blood.
1. A convenient spectrophotometric assay for the determination of l-ergothioneine in solution including deproteinized blood haemolysate was developed. 2. The method consists of deproteinization by heat precipitation and Cu(2+)-catalysed oxidation of thiols such as glutathione and of l-ascorbic acid, both in alkaline media, and titration of l-ergothioneine (which is not oxidized under these cond...
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One of the difficulties associated with research on ergothioneine has been the lack of completely reliable methods for the quantitative determination of the compound in blood and other tissues. Ergothioneine was originally discovered to be present in the red blood cell by Benedict et al. (1, 2) and by Hunter and Eagles (3, 4), some 25 years ago, because of its reducing action on the arsenophosp...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1974
ISSN: 0264-6021
DOI: 10.1042/bj1390237